After opening Ohio Valley Conference play with three-straight wins, the Colonels ran into a match-up with one of the most physical teams in the league.
They weren't able to rise to the challenge.
EKU shot just 28 percent from the field - including 2-of-15 from three-point range - in a loss on the road at Austin Peay last Saturday.
The Colonels faced another tough, defensive-focused team on Thursday at McBrayer Arena.
And once again, they struggled.
EKU (4-5, 3-2 OVC) had 17 turnovers and suffered a 74-60 loss to Jacksonville State (5-2, 2-1). Alice Recanati had 14 points and Jayla Johnson had 12 points as the Colonels shot 40.7 percent.
"It was a tough one," EKU coach Samantha Williams said. "JSU is one of the better teams in this league."
JSU came into the game second in the OVC in field goal percentage defense (35.9) while leading the league in scoring defense (60.3 points a game).
EKU took a quick 5-2 lead, but trailed 35-26 at halftime after hitting just 10-of-32 field goal attempts.
"They are a physical team, like Austin Peay, and that bothered us," Williams said. "With our youth and international players, we haven't experienced that kind of play a lot. They will have to get used to it."
JSU out-rebounded EKU 36-34, including a 12-8 advantage on the offensive boards. The Gamecocks had 12 second-chance points and 44 points in the paint.
EKU got Johnson back in the line-up after missing two games with an injury. The senior forward transfer from Texas State came off the bench and hit 5-of-12 field goals and had two rebounds.
Recanati was 4-of-5 from the field and had a team-best six rebounds, Anysa Jordan had 10 points, but was 4-of-11 from the field.
Kendall Wingler had 11 points and six rebounds as well.
Clara Rosini had six points.
JSU had four players in double figures. Taylor Hawks had 13 points, while Kennedy Griffin, Kiana Johnson and Yamia Johnson each had 13 points.
The Gamecocks shot 44 percent from the field, including 4-of-20 from three-point range. They scored 27 points off turnovers.
EKU is set to return to action Saturday at home against Tennessee Tech.