The Lady Indians finished the season ranked second in the Kentucky Golf Coaches Association state rankings.
They won't, however, get a chance to compete next week in the Kentucky High School Athletic Association State Tournament in Bowling Green.
Madison Central (324) finished second on Monday at the Region 9 Tournament in Keene Trace Golf Club.
And under new KHSAA regulations, only one team from each region advances to the state tournament, along with seven individuals not on the winning team.
Lexington Christian Academy took the title with a score of 314.
Central's Mattie Neeley and Reagan Clark will advance to the state tournament, but their teammates will not.
"Every other team that finished second at each region can feel the same way," Central coach Tennye Ohr said of the disappointment of her team not advancing to the state tournament. "I know the effort and the heart that these girls have put into this season. It's just sad."
Neeley finished in a tie with LCA's Laney Frye for the individual title after both shot a 71.
The senior beat Frye and two other players in a playoff to win the KGCA All-State Championship last weekend.
This time around, the LCA standout come up big on the first extra hole.
After Neeley went into the bunker, Frye delivered an eagle putt.
The Central senior, who was part of the school's 2015 state title team, moves on to the KHSAA Championships, where she will certainly be considered one of the favorites.
"She's had an amazing run the past two weeks," Ohr said of the senior who has committed to EKU. "That will set her up well for next week."
Clark shot an 81, which was tied for fifth, securing a spot at the state tournament.
Elizabeth Eberle shot an 82 and finished in a tie for seventh. She had to go to a playoff with Maddie Cecil of Dunbar for the final at-large spot in Bowling Green.
Cecil won on the first extra hole.
"I'm very happy very Mattie and I'm very happy for Reagan," Ohr said. "I wish Elizabeth had gotten there too. It's so frustrating."
The Lady Indians participated in 12 tournaments as a team this season. They had three wins, six runner-up finishes and were third three times.
"It hurts," Ohr said. "But, it will be fine."
Model Laboratory finished eighth overall after shooting a 386.
Lydia Harrel had a team-best 88, while Natalie Knecht shot a 93.
Allie Shaffer fired a 100, Lesley Durr shot a 105 and Amelia Harrel finished with a 111.
Madison Southern's Kasey Cameron shot a 97 and teammate Aysia Brock shot a 119.
The KHSAA Girls Golf State Championship is set for Oct. 7 through 9 at the Bowling Green Club.